Measurement

Part:BBa_K3228074

Designed by: Maurice Mager   Group: iGEM19_Marburg   (2019-10-15)


pMC_1_002_characterization_RBS_S.e.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3526
    Illegal PstI site found at 6061
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 427
    Illegal NheI site found at 450
    Illegal PstI site found at 6061
    Illegal NotI site found at 2328
    Illegal NotI site found at 8065
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7224
    Illegal XhoI site found at 3576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3526
    Illegal PstI site found at 6061
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3526
    Illegal PstI site found at 6061
    Illegal NgoMIV site found at 2149
    Illegal NgoMIV site found at 2375
    Illegal NgoMIV site found at 2517
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range BBa_K3228073 to BBa_K3228075 as well as BBa_K3228090) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria BBa_K3228069 and are therefore the only MoClo based vectors for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder (compare BBa_K3228060) for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization.

Figure 1: Design of the standardized measurement vectors for promoter and RBS BioBrick characterization

In our resultswe show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility. For greater comparability across other data sets we decided to use the same Biobricks as in measuring the toolbox for Vibrio natriegens in the last year.

Compability

These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Available standard measurement vectors

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Categories
Parameters
None